Leandro Paredes was once again benched for Moise Kean against Empoli, which surprised Juventus coach Max Allegri.
At 19:45 UK time, it begins at the Allianz Juventus Stadium in Turin (18.45 GMT).
The Bianconeri reversed the trend with a spirited 1-0 victory over local rivals Torino in the Derby della Mole, but they are still 10 points behind Serie A leaders Napoli and are in danger of being knocked out of the Champions League early.
Angel Di Maria, Mattia De Sciglio, Federico Chiesa, Paul Pogba, and Kaio Jorge are still absent, but they will be in Portugal on Tuesday to face Benfica, the co-leaders of their group.
When Kean was unexpectedly chosen over Arkadiusz Milik once more after the Torino game, the coach was questioned as to why Paredes had been benched for the third straight game.
“Kean is in good shape, so is Milik, so I simply made a choice to make the most of Kean’s form, as he played well against Torino.
“As for Paredes, we’ve got a game coming up on Tuesday, Empoli are a side who press hard and run a lot, so for 60-70 minutes we need to be on the same level of intensity.”
What can Juventus learn from its challenging start to this season, according to Allegri?
“The lessons Juventus need to learn is that to win games, we need to have the attitude of a team, without being hasty or rushing to secure the victory early, but remain consistent over the 90 minutes.”